In 1945, Yoshika Miyafuji, who lost her witch powers during the Strike Witches' last assignment, has been studying to become a doctor. Shizuka Hattori, one of her cadets in the Imperial Fuso Navy, then arrives to deliver a message: Yoshika is to be transferred for study abroad in Europe. When their enemy reappears more powerful than ever, the Strike Witches must form up once again. But can they do it without Yoshika Miyafuji?